The airline will launch flights to Southwest Florida International later this month.
This new flight follows the reintroduction of Air Berlin’s two weekly flights between Munich and the US holiday destination earlier this year.
As well as making it easier for passengers in Europe to reach Florida, the new flights will allow travellers from the US to access other cities in the Air Berlin network.
From Dusseldorf and Munich, passengers will be able to connect with flights to Berlin, Hamburg, Vienna, Zurich and London.
"We are excited that Air Berlin has brought back the popular Munich flights and increased service to Düsseldorf in time for the busy summer travel season," said Robert M Ball, executive director of the Lee County Port Authority, which operates South Florida International.
"Air Berlin’s extensive network allows passengers to travel onward from Dusseldorf or Munich to many German airports and top holiday destinations in Europe."
Cheap flights to Florida were recently introduced by Canadian airline Zoom. The carrier added flights from London Gatwick to Fort Lauderdale as part of its summer expansion plans.