The carrier will run flights between Cork, Ireland and Prestwick Airport in Glasgow from October 28th.
Aer Arann’s move follows an announcement last week that Ryanair will no longer be operating flights on this route.
Fergal Barry, Aer Arann’s commercial director, explained that the airline was keen to ensure that customers did not suffer a break in service on the route.
He said: "This is an excellent opportunity for Aer Arann to take over an existing route and develop it by offering our unique brand of quality service.
"We’re also very happy to ensure there is no break in service for regular customers as we introduce a second Scottish service from Cork."
The airline was recently awarded the Public Service Obligation route between Knock and Dublin, which will begin on October 1st.
Aer Arann will operate two daily flights between the destinations, with a early morning flight and evening return service.
This is an extension on a previous contract for Aer Arann, which had been responsible for flights on the route until July 2008.