Maiden flight EY294 took off from the United Arab Emirates at 10:05 (local time) last Thursday (May 7th) before touching down in Astana at 16:35 (local time).
An Airbus A319 will operate flights between the two cities on a once-a-week basis.
Commenting on the new route, an Etihad Airways statement said: "Astana is the second destination in Kazakhstan to be served by Etihad and the airline’s 52nd across its expanding global flight network.
"The start of the new service follows the launch by Etihad in December 2008 of flights to Almaty, Kazakhstan’s largest city."
In order to accommodate the new route, Etihad is reducing its four weekly flights to Almaty to three.
Last month, the Etihad Airways began daily flights to Melbourne’s Tullamarine Airport from Abu Dhabi.
The route is Etihad’s third Australian destination after it introduced flights to Sydney and Brisbane.