Chicago will become Etihad’s second US destination after it introduced a daily flight from the United Arab Emirates (UAE) to New York.
The flight will initially operate three times per week, although a daily service will be introduced from October 1st.
James Hogan, Etihad’s chief executive, said the new flight would be a "very proud moment" in the airline’s history.
"The new service will provide a daily flight for business and leisure travellers as well as enhance commercial and cultural ties not only between Abu Dhabi and Chicago but also between the UAE and US," he said.
Over the course of 2009, Etihad will also introduce flights to Melbourne, Athens and Istanbul.
The airline will fly to 55 cities around the world by the close of this year, with the carrier also counting Toronto as part of its north American network.
Etihad announced last month that it had signed a new codeshare agreement with Bangkok Airways.