This year’s summer schedule will begin at the start of May and run until the middle of October, with the number of flights to Canada increasing from three to four a week.
A total of 156 flights will be operated between the two cities this summer, representing a year-on-year increase of 44 percent.
Commenting on the increase, a Czech Airlines press release said: "The new model of operating to North America, launched last year, when Czech Airlines only focused on seasonal flights to Toronto, has increased profitability and the seat-load factor."
The airline has said that it expects almost 30,000 passengers will use the route over the course of the summer.
Last year, a little over 19,000 passengers used the route.
Czech Airlines announced earlier this month that the number of people that have signed up to its frequent flyer programme has risen by 28 percent.