The airline will introduce the direct, twice-weekly service to the sought-after Thai resort on November 14th.
Flights will depart on Tuesdays and Saturdays at 10:35 (local time), while incoming flights will arrive on Wednesdays and Sundays at 21:35 (local time).
It is expected that the flights will prove popular as a result of increased demand among western Australians for cheaper and convenient travel options to Thailand.
Commenting on the route, Perth Airport chief executive officer Brad Geatches said: "The introduction of Pacific Blue's new service to Phuket will be perfect for those looking to escape to south-east Asia.
"This service offers more travel options to western Australians and highlights the popularity of this region."
Pacific Blue Airlines is part of the Virgin Blue Group, which recently increased its presence in Asia with the introduction of daily flights to Bali.
From October 2nd, cheap flights carrier Jetstar Airways will launch daily flights to Sydney from the Perth.
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